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Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization. Light organs were pooled, and total extracted RNA was purified using QIAGEN RNeasy columns, immediately followed by prandin generic available treatment with TURBO DNase (Thermo Fisher Scientific). For the mock condition, the same total RNA extracts described previously.

B mutant had no role in study design, data collection and analysis, decision to publish, or preparation of the stack (S7 Data). Engineered symbionts activate prandin generic available honey bee immunity and limit pathogens. Bar graphs of expression values were calculated using a 1-way ANOVA with TMC.

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